Article Essay Example | Topics and Well Written Essays - 1500 words - 1

Article - Essay Example(Krawczyk, M.et.al, 2008, p. 136). The tuition concludes that the CC- genotype, BTH and genotype correlate perfectly, and the genetic test provides an unambiguous result. In both positive individual with a electronegative genetic test there is good reason to suspect secondary causes of lactase deficiency. (Krawczyk, M.et.al, 2008, p.138).In the study 58 consecutives patients are chosen (25 males, 33 females median age 41 years, range 18 82 years) recruited prospectively between April 2005 and July 2007. (Krawczyk, M.et.al, 2008, p.137). These patients were referred by the department with non-specific gastrointestinal symptoms consistent with lactose intolerance (i.e., bloating, abdominal pain, diarrhea).All participants signed an sensible consent form, and the study was conducted according to a study design approved by the local honorable committee. (Krawczyk, M.et.al, 2008, p.137).The method in genetic test includes the use of a Peripheral venous blood samp les for desoxyribonucleic acid testing were obtained from all patients. DNA was isolated using the DNeasy Blood & Tissue kit (Qiagen, Hilden, Germany). The genotyping procedure consisted of polymerase fibril reaction (PCR) amplification and SNP detection of the -13,910CT variant using SNaPshot minisequencing (Applera, Norwalk,CT). (Krawczyk, M.et.al, 2008, p.136).For Hydrogen breathe test (BTH), it was performed subsequently at least 12 hours overnight fasting. All patients were obliged to restrain from cigarette smoking forrader the test. Additionally, individuals who underwent colonoscopy or were taking any antibiotics in the fortnight before the test were excluded from the study. The test was performed after using up of 50 g of lactose diluted in 300 ml of water. The amount of exhaled hydrogen was thrifty in parts per million (ppm) before lactose ingestion (baseline), every 10 minutes during the commencement ceremony hour and every 20 minutes